Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet: Keratinocyte panel

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Concentration Assay, Staining

Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Recombinant, Staining, Cream, Software, Flow Cytometry

Journal: Cell Death and Differentiation

Article Title: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition

doi: 10.1038/s41418-025-01502-x

Figure Lengend Snippet: A Treatment scheme for the p53-proficient AOM/DSS mouse model. After tumor visualization by repeat colonoscopy and tumor scoring for at least one S2-sized tumor and three S1-sized tumors per mouse, mice were treated with single drugs or combination treatment for 19 days. 4 h after the final dose, mice were dissected and analyzed. B , C Representative images of entire resected colons ( B ) and H&E-stained colon sections ( C ) from single or combination-treated C57BL6/J mice at endpoint as described in ( A ). Mice received 50 mg/kg RG-7388 orally 5× per week, or 50 mg/kg Ganetespib intravenously 2× per week, or both. B scale bars, 5 mm. C ×20 magnification, scale bars, 1 cm. D Tumor surface area from AOM/DSS mice that had received single or combination treatment for 19 days as in ( A ). Cross-sections of Swiss roles were H&E stained (Supplementary Fig. ). Tumor areas of all tumors per H&E-stained Swiss role were measured using ImageJ. Bar, mean. vehicle n = 35 tumors from 5 mice; RG-7388 and Ganetespib n = 33 tumors from 6 mice each; combination n = 45 tumors from 9 mice. E , F GSEA hallmark analysis of RNAseq data from HCT116 cells treated with drug combination or single drugs for 24 h. Enriched pathways are plotted according to NES. Gray bars: Enrichment in ( F ) RG-7388-only or ( G ) Ganetespib-only versus DMSO controls. Red bars: further enrichment with combination treatment relative to single treatment. NES: normalized enrichment score. G – I Multiplex immunohistochemistry of p53-proficient tumors from AOM/DSS mice receiving drug treatments for 19 days as in ( A ). G Representative images of indicated groups. Ly6G staining represents neutrophils, and panCytokeratin represents tumor-epithelial cells. DAPI counter staining. Scale bars, 50 µm. H Quantification of Ly6G + CD11b + F4/80− neutrophils using the “Vectra Polaris” platform and the inForm Advanced Image Analysis software. n = 5 mice per group. All tumors from stained Swiss roles were analyzed for the indicated treatment groups. I Plot of the neutrophil-lymphocyte-ratio (NLR) as a marker of an inflammatory response. A low NLR indicates an inflammatory response. J , K Multiplex immunohistochemistry of tumor-bearing AOM/DSS mice who received a short drug treatment of 6 days. Quantification of Ly6G + CD11b + F4/80− neutrophils of the indicated treatment groups as in ( H ). DMSO, Ganetespib, and RG-7388 mice n = 3 each, combination-treated mice n = 4. C , H – K Mean ± SEM. Student’s t -test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; ns not significant. Ganet: Ganetespib, RG: RG-7388.

Article Snippet: The following primary rabbit antibodies were used for staining: CD4 ( CST25229 ; 1:200), CD8 ( CST98941 ; 1:400), F4/80 (CST 70076; 1:1000), and Ly6G (CST 87048; 1:1000) were purchased from Cell Signaling Technology ® ; CD11b ( Ab133357 ; 1:3000) from Abcam ® ; PanCytokeratin (NBP3-07280; 1:1000) from Novus Biologicals ® .

Techniques: Staining, Multiplex Assay, Immunohistochemistry, Software, Marker